当神经科学邂逅组织透明化——会独创怎样的故事？

howing labeled axons in absence of β-amyloid plaques. (F) Magnified region from (D) showing dystrophic axons surrounding labeled β-amyloid plaques. (G) Maximum intensity projection of optical section (1mm) from the cortex of an 11-month-old 2xTgAD mouse brain labeled for plaques (anti-b amyloid anti-bodies) and vasculature. (H)Maximum intensity projection of optical section (1mm) from the cortex and hippocampus of an 8-month-old 2xTgAD mouse brain labeled for plaques (Congored) and vasculature.

Chung等人通过CLARITY核心技术获得表面而完整的人体内神经干，借助于Thy1–eYFP回波实现了对人体内神经干皮质展开短距离投射、神经部元件、细胞核父子关系、亚细胞核本体、酶质复合物、 多肽和中枢神经部的高分辨率。展现出了神经干里面多样的精细相互连接和分子本体。

Intact adult mouse brain imaging

a, Cajal quote before CLARITY. b, Cajal quote after CLARITY: Thy1–eYFP line-H mouse brain after hydrogel–tissue hybridization, ETC and refractive-index matching. c, Fluorescence image of brain depicted in b. d, Dorsal aspect is imaged, then brain is inverted and ventral aspect imaged. e, Three-dimensional rendering of clarified brain imaged；f, Non sectioned mouse brain tissue showing cortex, hippocampus and thalamus；g–l, Optical sections from f showing negligible resolution；m, Cross-section of axons in clarified Thy1–channelrhodopsin2 (ChR2)–eYFP striatum；n, Dendrites and spines of neurons in clarified Thy1–eYFP line-H cortex.

MURAKAMI等人应用层面CUBIC-X表面并膨大人体内神经干，在亚细胞核低水后平对整个人体内神经干展开高分辨率，并绘制造出了一张人体内神经干图谱。他们选用化学方法有标识了神经干里面的每个细胞核，然后在神经干表面化的同时将其宽度扩大了十倍，借助于精准高分辨率核心技术对皮质展开了缩放重建，将近近7200万个细胞核。

Construction of a single-cell-resolution mouse brain atlas (CUBIC-Atlas)

a, Overview of construction of the CUBIC-Atlas. b, The CUBIC-Atlas. Horizontal, sagittal and coronal view of single-plane images (left) and volume-rendered images (right) of the CUBIC-Atlas. c–j, Major anatomical areas in the CUBIC-Atlas. k, Overview of whole-brain cell counting in C57BL/6N 8-week-old male mice. l, Cell numbers in each brain area.

总之，一个组织表面核心技术结合光学高分辨率核心技术，更大推展了全身或全肾脏高分辨率的数据流，借以加强对完整动物系统的整体理解，为神经部科研究课题提供了极其生动的讯息，是神经部科研究课题层面强有利的辅助工具。

现有一个组织表面化方法有有油性，基于水后气相和水后性的一个组织表面化方法有。其里面油性一个组织表面化方法有主要有BABB, 3DISCO, uDISCO，FDISCO和 PEGASOS等，基于水后气相的表面化方法有有CLARITY和SHIELD，水后性一个组织表面化方法有有CLearT/CLearT2,SeeDB, FRUIT, Scale和CUBIC等方法有。而锘洋最新制造的表面化醛盒依托测样服务里面受益到的多样而有价值的经验，对各种表面化方法有展开测试和比较后对醛配方展开了巧妙优化，使其在一个组织荧光保护、抽取特征保持稳定、提高民间一个组织荧光等方面体现造出非常优异的安全性，是研究课题神经部科的不二之选。

参考文献：

1. Ertürk A, Mauch CP, Hellal F, Förstner F, Keck T, Becker K, Jährling N, Steffens H, Richter M, Hübener M, Kramer E, Kirchhoff F, Dodt HU, Bradke F. Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury. Nat Med. 2011 Dec 25;18(1):166-71. doi: 10.1038/nm.2600. PMID: 22198277.

2. Liebmann T, Renier N, Bettayeb K, Greengard P, Tessier-Lavigne M, Flajolet M. Three-Dimensional Study of Alzheimer's Disease Hallmarks Using the iDISCO Clearing Method. Cell Rep. 2016 Jul 26;16(4):1138-1152. doi: 10.1016/j.celrep.2016.06.060. Epub 2016 Jul 14. PMID: 27425620; PMCID: PMC5040352.

3. Chung K, Wallace J, Kim SY, Kalyanasundaram S, Andalman AS, Davidson TJ, Mirzabekov JJ, Zalocusky KA, Mattis J, Denisin AK, Pak S, Bernstein H, Ramakrishnan C, Grosenick L, Gradinaru V, Deisseroth K. Structural and molecular interrogation of intact biological systems. Nature. 2013 May 16;497(7449):332-7. doi: 10.1038/nature12107. Epub 2013 Apr 10. PMID: 23575631; PMCID: PMC4092167.

4. Murakami TC, Mano T, Saikawa S, Horiguchi SA, Shigeta D, Baba K, Sekiya H, Shimizu Y, Tanaka KF, Kiyonari H, Iino M, Mochizuki H, Tainaka K, Ueda HR. A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing. Nat Neurosci. 2018 Apr;21(4):625-637. doi: 10.1038/s41593-018-0109-1. Epub 2018 Mar

5. PMID: 29507408.5. Tian T, Yang Z, Li X. Tissue clearing technique: Recent progress and biomedical applications. J Anat. 2021 Feb;238(2):489-507. doi: 10.1111/joa.13309. Epub 2020 Sep 16. PMID: 32939792; PMCID: PMC7812135.

。

成都风湿医院哪最好
驻马店哪里无痛人流好
北京妇科哪家医院最好
佛山男科检查费用
汉中白癜风医院去哪家好